ABSTRACT
Objective To explore the effect of platelet-rich plasma (PRP) on the generation of reactive oxygen species (ROS) and the phenotypes of photo-aging fibroblasts.Methods A photoaging cell model by repeating UVB irradiation was treated using appropriate concentration of PRP;Cell morphology and the rate of aging dying were observed under inverted microscope 24 hours later after establishment of the cell model;The expression of ROS between experimental and control group was detected using fluorescence microscope after single UVB irradiation.The relative intensity of fluorescence was analyzed using flow cytometry.Results PRP could ameliorate the large and sprawl appearance of photoaging fibroblasts obviously,reduce the generation of ROS as well as decrease the relative intensity of ROS.Conclusions PRP can decrease the level of intracellular oxidative stress caused by UVB irradiation,reduce the generation of ROS and ameliorate the senescence-like phenotypes of pho toaging fibroblasts.
ABSTRACT
Objective To investigate the end-digit preference and related factors in blood pressure measurement among inpatients.Methods A cross-sectional study was conducted on the end digits of blood pressure measurement during the admission of patients in a tertiary general hospital of shanghai in 2010,and the related factors were analyzed with the logistic regression.Results The average blood pressure in records of 2276 patients was (125 ± 14) mm Hg(1 mm Hg =0.133 kPa) in systolic and (77 ±9) mm Hg in diastolic.End-digital zero preference accounted for 1874 records (82.3％) of systolic and 1859 records (81.7％) of diastolic readings,which were significant different to the expected frequency of 20％ (P ＜ 0.001).Logistic analysis showed that admission of patients not in winter (OR =1.270,OR =1.270),patients in surgical department (OR =1.619,OR =2.045),patients with no history of hypertension (OR =1.432,OR =1.310)were the risk factors of end-digital zero preference in systolic and diastolic pressure measurement.Non-elderly patients (OR =1.288) and patients with normal heart rate(OR =1.823) were related to zero preference diastolic pressure measurement.Conclusions Blood pressure measurement of inpatient displays marked zero end-digit preference.Doctors tend to end in zero when taking blood pressure in some special types of patients.